נגישות
menu      
חיפוש מתקדם
תחביר
חפש...
הספר "אוצר וולקני"
אודות
תנאי שימוש
ניהול
קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Characterization of RR-1 and RR-2 cuticular proteins from Myzus persicae
Year:
2007
Authors :
דומברובסקי, אביב
;
.
סובולב, אירנה
;
.
צ'חנובסקי, נור
;
.
רקח, בנימין
;
.
Volume :
146
Co-Authors:
Dombrovsky, A., The Volcani Center, Department of Virology, Bet Dagan, 50250, Israel
Sobolev, I., The Volcani Center, Department of Virology, Bet Dagan, 50250, Israel
Chejanovsky, N., Entomology, The Volcani Center, Bet Dagan, 50250, Israel
Raccah, B., The Volcani Center, Department of Virology, Bet Dagan, 50250, Israel
Facilitators :
From page:
256
To page:
264
(
Total pages:
9
)
Abstract:
A cDNA library for Myzus persicae has served to identify sequences coding for cuticular proteins (CPs) with RR-1 and RR-2 consensus. Two putative CPs showed a common RR-2 chitin binding domain (CBD) but differed in their C and N terminals. Two other predicted CPs showed a typical RR-1 CBD but differed in size and sequence of the C and N terminals. An additional sequence encoding for a protein that showed terminal amino acid repeats similar to those of putative CPs from M. persicae, but lacked the R & R consensus, was also described. A comparison of the sequences obtained from the cDNA library with those attained from the genomic DNA, confirmed their identity as cuticular proteins genes. Presence of introns was revealed in the Mpcp4 and Mpcp5 genes coding for CPs with an RR-1 consensus. The Mpcp4 has a single large intron, while the Mpcp5 has two shorter ones. Introns were not found in the Mpcp2 and Mpcp3 genes encoding for CPs with RR-2 consensus. Differences were also noticed for 3′ UTR and 5′ UTR of both the RR-1 and RR-2 CPs. CPs genes were expressed in bacteria, and the resulting protein was identified as a CP by amino acid sequencing. © 2006 Elsevier Inc. All rights reserved.
Note:
Related Files :
Animals
Base Sequence
Gene
gene expression
Myzus persicae
RR-2
unclassified drug
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/j.cbpb.2006.11.013
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
22445
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:51
You may also be interested in
Scientific Publication
Characterization of RR-1 and RR-2 cuticular proteins from Myzus persicae
146
Dombrovsky, A., The Volcani Center, Department of Virology, Bet Dagan, 50250, Israel
Sobolev, I., The Volcani Center, Department of Virology, Bet Dagan, 50250, Israel
Chejanovsky, N., Entomology, The Volcani Center, Bet Dagan, 50250, Israel
Raccah, B., The Volcani Center, Department of Virology, Bet Dagan, 50250, Israel
Characterization of RR-1 and RR-2 cuticular proteins from Myzus persicae
A cDNA library for Myzus persicae has served to identify sequences coding for cuticular proteins (CPs) with RR-1 and RR-2 consensus. Two putative CPs showed a common RR-2 chitin binding domain (CBD) but differed in their C and N terminals. Two other predicted CPs showed a typical RR-1 CBD but differed in size and sequence of the C and N terminals. An additional sequence encoding for a protein that showed terminal amino acid repeats similar to those of putative CPs from M. persicae, but lacked the R & R consensus, was also described. A comparison of the sequences obtained from the cDNA library with those attained from the genomic DNA, confirmed their identity as cuticular proteins genes. Presence of introns was revealed in the Mpcp4 and Mpcp5 genes coding for CPs with an RR-1 consensus. The Mpcp4 has a single large intron, while the Mpcp5 has two shorter ones. Introns were not found in the Mpcp2 and Mpcp3 genes encoding for CPs with RR-2 consensus. Differences were also noticed for 3′ UTR and 5′ UTR of both the RR-1 and RR-2 CPs. CPs genes were expressed in bacteria, and the resulting protein was identified as a CP by amino acid sequencing. © 2006 Elsevier Inc. All rights reserved.
Scientific Publication
You may also be interested in