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Plant Science
Ezra, D., Department of Plant Sciences, Montana State University, 206 Ag BioSciences Bldg., Bozeman, MT 59717, United States
Jasper, J., Department of Chemistry, Montana State University, Bozeman, MT 59717, United States
Rogers, T., Department of Chemistry, Montana State University, Bozeman, MT 59717, United States
Knighton, B., Department of Chemistry, Montana State University, Bozeman, MT 59717, United States
Grimsrud, E., Department of Chemistry, Montana State University, Bozeman, MT 59717, United States
Strobel, G., Department of Plant Sciences, Montana State University, 206 Ag BioSciences Bldg., Bozeman, MT 59717, United States
Muscodor albus is an endophytic fungus that produces volatile organic compounds (VOCs) that both inhibit and kill other microorganisms. This fungus is now being used to treat human wastes and disinfest soils of plant disease causing organisms. The development of a method to accurately determine the quantity and quality of volatiles being emitted by this organism is critical for optimizing its application as an antimicrobial agent. Proton transfer reaction-mass spectrometry (PTR-MS) was used to monitor the concentration of VOCs emitted by M. albus. This on-line technique is fast, accurate and provides data at the detection limits of ppb. Production of VOCs is temperature dependent with decreased gas production occurring at higher temperatures. The technique was also applied to soils containing M. albus along with the plant pathogen Pythium ultimum and it was possible to successfully monitor VOC production in situ. © 2004 Elsevier Ireland Ltd. All rights reserved.
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Proton transfer reaction-mass spectrometry as a technique to measure volatile emissions of Muscodor albus
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Ezra, D., Department of Plant Sciences, Montana State University, 206 Ag BioSciences Bldg., Bozeman, MT 59717, United States
Jasper, J., Department of Chemistry, Montana State University, Bozeman, MT 59717, United States
Rogers, T., Department of Chemistry, Montana State University, Bozeman, MT 59717, United States
Knighton, B., Department of Chemistry, Montana State University, Bozeman, MT 59717, United States
Grimsrud, E., Department of Chemistry, Montana State University, Bozeman, MT 59717, United States
Strobel, G., Department of Plant Sciences, Montana State University, 206 Ag BioSciences Bldg., Bozeman, MT 59717, United States
Proton transfer reaction-mass spectrometry as a technique to measure volatile emissions of Muscodor albus
Muscodor albus is an endophytic fungus that produces volatile organic compounds (VOCs) that both inhibit and kill other microorganisms. This fungus is now being used to treat human wastes and disinfest soils of plant disease causing organisms. The development of a method to accurately determine the quantity and quality of volatiles being emitted by this organism is critical for optimizing its application as an antimicrobial agent. Proton transfer reaction-mass spectrometry (PTR-MS) was used to monitor the concentration of VOCs emitted by M. albus. This on-line technique is fast, accurate and provides data at the detection limits of ppb. Production of VOCs is temperature dependent with decreased gas production occurring at higher temperatures. The technique was also applied to soils containing M. albus along with the plant pathogen Pythium ultimum and it was possible to successfully monitor VOC production in situ. © 2004 Elsevier Ireland Ltd. All rights reserved.
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