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Plant Science
Zhang, J., College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China
Ma, H., College of Agriculture and Biotechnology, China Agricultural University, Beijing, China
Chen, S., College of Agriculture and Biotechnology, China Agricultural University, Beijing, China
Ji, M., College of Agriculture and Biotechnology, China Agricultural University, Beijing, China
Perl, A., Department of Fruit Tree Breeding and Molecular Genetics, Agricultural Research Organization, The Volcani Center, Israel
Kovacs, L., Department of Agriculture, Missouri State University, United States
Chen, S., College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China, Key Lab of Functional Dairy Science, Chinese Ministry of Education, Beijing, 100083, China
Co-cultivation of grape embryogenic callus (EC) and somatic embryos with Agrobacterium often leads to necrogenesis, which manifests in tissue browning and subsequent cell death. Interestingly, however, co-cultivation of grape non-embryogenic callus (NEC) does not display any visible symptoms. This paper compares the proteome of EC and NEC of Vitis vinifera L. cv. Cabernet Sauvignon to investigate the underlying mechanism. Analysis of 2-DE gels revealed 1180 spots, with 154 of them detected as differentially responsive proteins in EC versus NEC. Of these, the identity of 108 proteins was determined, with 28 proteins falling in the stress-response category. Those exclusively or predominantly identified in EC were iron-deficiency-responsive protein, acidic ascorbate peroxidase and isoflavone reductase-like protein, and those that were exclusively or predominant in NEC were basic ascorbate peroxidase, catalase, calcineurin B-like protein, 1,3-β-glucanase and cyclin-dependent kinase A1. The abundant protein PR-10 was at a remarkably lower level in EC than in NEC. These results demonstrated that different stress response pathways were activated in EC from those in NEC. A number of additional differentially expressed proteins involved in various functional categories were also identified. © 2009 Elsevier Ireland Ltd.
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Stress response proteins' differential expression in embryogenic and non-embryogenic callus of Vitis vinifera L. cv. Cabernet Sauvignon-A proteomic approach
177
Zhang, J., College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China
Ma, H., College of Agriculture and Biotechnology, China Agricultural University, Beijing, China
Chen, S., College of Agriculture and Biotechnology, China Agricultural University, Beijing, China
Ji, M., College of Agriculture and Biotechnology, China Agricultural University, Beijing, China
Perl, A., Department of Fruit Tree Breeding and Molecular Genetics, Agricultural Research Organization, The Volcani Center, Israel
Kovacs, L., Department of Agriculture, Missouri State University, United States
Chen, S., College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, China, Key Lab of Functional Dairy Science, Chinese Ministry of Education, Beijing, 100083, China
Stress response proteins' differential expression in embryogenic and non-embryogenic callus of Vitis vinifera L. cv. Cabernet Sauvignon-A proteomic approach
Co-cultivation of grape embryogenic callus (EC) and somatic embryos with Agrobacterium often leads to necrogenesis, which manifests in tissue browning and subsequent cell death. Interestingly, however, co-cultivation of grape non-embryogenic callus (NEC) does not display any visible symptoms. This paper compares the proteome of EC and NEC of Vitis vinifera L. cv. Cabernet Sauvignon to investigate the underlying mechanism. Analysis of 2-DE gels revealed 1180 spots, with 154 of them detected as differentially responsive proteins in EC versus NEC. Of these, the identity of 108 proteins was determined, with 28 proteins falling in the stress-response category. Those exclusively or predominantly identified in EC were iron-deficiency-responsive protein, acidic ascorbate peroxidase and isoflavone reductase-like protein, and those that were exclusively or predominant in NEC were basic ascorbate peroxidase, catalase, calcineurin B-like protein, 1,3-β-glucanase and cyclin-dependent kinase A1. The abundant protein PR-10 was at a remarkably lower level in EC than in NEC. These results demonstrated that different stress response pathways were activated in EC from those in NEC. A number of additional differentially expressed proteins involved in various functional categories were also identified. © 2009 Elsevier Ireland Ltd.
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