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פותח על ידי קלירמאש פתרונות בע"מ -
Comparative mapping of ZYMV resistances in cucumber (Cucumis sativus L.) and melon (Cucumis melo L.)
Year:
2004
Source of publication :
Theoretical and Applied Genetics
Authors :
קציר, נורית
;
.
Volume :
109
Co-Authors:
Park, Y., Department of Horticulture, University of Wisconsin, 1575 Linden Drive, Madison, WI 53706, United States
Katzir, N., Department of Vegetable Crops, Agricultural Research Organization, Newe Ya'ar Research Center, PO Box 1021, Ramat Yishay, 30095, Israel
Brotman, Y., Department of Vegetable Crops, Agricultural Research Organization, Newe Ya'ar Research Center, PO Box 1021, Ramat Yishay, 30095, Israel, Faculty of Life Sciences, Bar-Ilan University, Ramat Gan, 52900, Israel
King, J., Seminis Vegetable Seeds, 37437 State Highway 16, Woodland, CA 95695, United States
Bertrand, F., Seminis Vegetable Seeds, Mas de Rouzel, Chemin des Canaux, 30900 Nîmes, France
Havey, M., Vegetable Crops Unit, Department of Horticulture, University of Wisconsin, 1575 Linden Drive, Madison, WI 53706, United States
Facilitators :
From page:
707
To page:
712
(
Total pages:
6
)
Abstract:
Zucchini yellow mosaic virus (ZYMV) routinely causes significant losses in cucumber (Cucumis sativus L.) and melon (Cucumis melo L.). ZYMV resistances from the cucumber population 'TMG1' and the melon plant introduction (PI) 414723 show different modes of inheritance and their genetic relationships are unknown. We used molecular markers tightly linked to ZYMV resistances from cucumber and melon for comparative mapping. A 5-kb genomic region (YCZ-5) cosegregating with the zym locus of cucumber was cloned and sequenced to reveal single nucleotide polymorphisms and indels distinguishing alleles from ZYMV-resistant (TMG1) and susceptible (Straight 8) cucumbers. A low-copy region of the YCZ-5 clone was hybridized to bacterial artificial chromosome (BAC) clones of melon and a 180-kb contig assembled. One end of this melon contig was mapped in cucumber and cosegregated with ZYMV resistance, demonstrating that physically linked regions in melon show genetic linkage in cucumber. However the YCZ-5 region segregated independently of ZYMV resistance loci in two melon families. These results establish that these sources of ZYMV resistances from cucumber TMG1 and melon PI414723 are likely nonsyntenic. © Springer-Verlag 2004.
Note:
Related Files :
biodiversity
chromosome mapping
Cucumis melo
genetic engineering
genetic markers
Genetics
עוד תגיות
תוכן קשור
More details
DOI :
10.1007/s00122-004-1684-y
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
30358
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:53
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Scientific Publication
Comparative mapping of ZYMV resistances in cucumber (Cucumis sativus L.) and melon (Cucumis melo L.)
109
Park, Y., Department of Horticulture, University of Wisconsin, 1575 Linden Drive, Madison, WI 53706, United States
Katzir, N., Department of Vegetable Crops, Agricultural Research Organization, Newe Ya'ar Research Center, PO Box 1021, Ramat Yishay, 30095, Israel
Brotman, Y., Department of Vegetable Crops, Agricultural Research Organization, Newe Ya'ar Research Center, PO Box 1021, Ramat Yishay, 30095, Israel, Faculty of Life Sciences, Bar-Ilan University, Ramat Gan, 52900, Israel
King, J., Seminis Vegetable Seeds, 37437 State Highway 16, Woodland, CA 95695, United States
Bertrand, F., Seminis Vegetable Seeds, Mas de Rouzel, Chemin des Canaux, 30900 Nîmes, France
Havey, M., Vegetable Crops Unit, Department of Horticulture, University of Wisconsin, 1575 Linden Drive, Madison, WI 53706, United States
Comparative mapping of ZYMV resistances in cucumber (Cucumis sativus L.) and melon (Cucumis melo L.)
Zucchini yellow mosaic virus (ZYMV) routinely causes significant losses in cucumber (Cucumis sativus L.) and melon (Cucumis melo L.). ZYMV resistances from the cucumber population 'TMG1' and the melon plant introduction (PI) 414723 show different modes of inheritance and their genetic relationships are unknown. We used molecular markers tightly linked to ZYMV resistances from cucumber and melon for comparative mapping. A 5-kb genomic region (YCZ-5) cosegregating with the zym locus of cucumber was cloned and sequenced to reveal single nucleotide polymorphisms and indels distinguishing alleles from ZYMV-resistant (TMG1) and susceptible (Straight 8) cucumbers. A low-copy region of the YCZ-5 clone was hybridized to bacterial artificial chromosome (BAC) clones of melon and a 180-kb contig assembled. One end of this melon contig was mapped in cucumber and cosegregated with ZYMV resistance, demonstrating that physically linked regions in melon show genetic linkage in cucumber. However the YCZ-5 region segregated independently of ZYMV resistance loci in two melon families. These results establish that these sources of ZYMV resistances from cucumber TMG1 and melon PI414723 are likely nonsyntenic. © Springer-Verlag 2004.
Scientific Publication
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