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Homeodomain leucine zipper proteins bind to the phosphate response domain of the soybean VspB tripartite promoter
Year:
2001
Authors :
צדקה, אבי
;
.
Volume :
125
Co-Authors:
Tang, Z., Dept. of Biochemistry and Biophysics, Texas A and M University, College Station, TX 77840, United States
Sadka, A., Dept. of Biochemistry and Biophysics, Texas A and M University, College Station, TX 77840, United States
Morishige, D.T., Dept. of Biochemistry and Biophysics, Texas A and M University, College Station, TX 77840, United States
Mullet, J.E., Dept. of Biochemistry and Biophysics, Texas A and M University, College Station, TX 77840, United States
Facilitators :
From page:
797
To page:
809
(
Total pages:
13
)
Abstract:
The soybean (Glycine max L. Merr. cv Williams 82) genes VspA and VspB encode vacuolar glycoprotein acid phosphatases that serve as vegetative storage proteins during seed fill and early stages of seedling growth. VspB expression is activated by jasmonates (JAs) and sugars and down-regulated by phosphate and auxin. Previous promoter studies demonstrated that VspB promoter sequences between -585 and -535 mediated responses to JA, and sequences between -535 and -401 mediated responses to sugars, phosphate, and auxin. In this study, the response domains were further delineated using transient expression of VspB promoter-β-glucuronidase constructs in tobacco protoplasts. Sequences between -536 and -484 were identified as important for phosphate responses, whereas the region from -486 to -427 mediated sugar responses. Gel-shift and deoxyribonuclease-I footprinting assays revealed four DNA-binding sites between -611 and -451 of the soybean VspB promoter: one in the JA response domain, two in the phosphate response domain, and one binding site in the sugar response domain. The sequence CATTAATTAG present in the phosphate response domain binds soybean homeodomain leucine zipper proteins, suggesting a role for these transcription factors in phosphate-modulated gene expression.
Note:
Related Files :
Base Sequence
Binding Sites
Glycine max
homeodomain protein
jasmonic acid
Sequence Homology, Amino Acid
עוד תגיות
תוכן קשור
More details
DOI :
10.1104/pp.125.2.797
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
30520
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:55
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Scientific Publication
Homeodomain leucine zipper proteins bind to the phosphate response domain of the soybean VspB tripartite promoter
125
Tang, Z., Dept. of Biochemistry and Biophysics, Texas A and M University, College Station, TX 77840, United States
Sadka, A., Dept. of Biochemistry and Biophysics, Texas A and M University, College Station, TX 77840, United States
Morishige, D.T., Dept. of Biochemistry and Biophysics, Texas A and M University, College Station, TX 77840, United States
Mullet, J.E., Dept. of Biochemistry and Biophysics, Texas A and M University, College Station, TX 77840, United States
Homeodomain leucine zipper proteins bind to the phosphate response domain of the soybean VspB tripartite promoter
The soybean (Glycine max L. Merr. cv Williams 82) genes VspA and VspB encode vacuolar glycoprotein acid phosphatases that serve as vegetative storage proteins during seed fill and early stages of seedling growth. VspB expression is activated by jasmonates (JAs) and sugars and down-regulated by phosphate and auxin. Previous promoter studies demonstrated that VspB promoter sequences between -585 and -535 mediated responses to JA, and sequences between -535 and -401 mediated responses to sugars, phosphate, and auxin. In this study, the response domains were further delineated using transient expression of VspB promoter-β-glucuronidase constructs in tobacco protoplasts. Sequences between -536 and -484 were identified as important for phosphate responses, whereas the region from -486 to -427 mediated sugar responses. Gel-shift and deoxyribonuclease-I footprinting assays revealed four DNA-binding sites between -611 and -451 of the soybean VspB promoter: one in the JA response domain, two in the phosphate response domain, and one binding site in the sugar response domain. The sequence CATTAATTAG present in the phosphate response domain binds soybean homeodomain leucine zipper proteins, suggesting a role for these transcription factors in phosphate-modulated gene expression.
Scientific Publication
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