Co-Authors:
Mayer, R.T., Veterinary Toxicology and Entomology Research Laboratory, Agricultural search Service, U.S. Department of Agriculture, College Station, TX 77840, United States
Svoboda, J.A., Insect Physiology Laboratory, Plant Protection Institute, Agricultural Research Service, Beltsville, MD 20705, United States
Abstract:
The possibility of quantitating insect ecdysones in situ on thin-layer Chromatographic plates was examined. Two approaches were evaluated: 1) the induction of ecdysone fluorescence by sulfurio acid treatments and 2) the fluorescence-quenching of fluorescent thin-layer Chromatographic plates by ecdysones. The fluorescence-quenching method was found to be most suitable and had a linear response range from 0.5 to 3 μg for α-ecdysone and 20-hydroxyecdysone. Fluorescence-quenching and high pressure liquid Chromatographic analyses obtained, from extracts of α-ecdysone 20-hydroxylase incubations gave similar results. New data concerning the acid-induced fluorescence of ecdysones showed α-ecdysone to be twice as fluorescent as 20-hydroxyecdysone. © 1978.
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