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פותח על ידי קלירמאש פתרונות בע"מ -
Distinguishing between Bread Wheat and Spelt Grains Using Molecular Markers and Spectroscopy
Year:
2019
Authors :
בונפיל, דוד
;
.
בן-דוד, רואי
;
.
נאשף, כמאל
;
.
צ'נדרסקר, קוטקוטה
;
.
קורזון, אריה
;
.
Volume :
67
Co-Authors:

Abbo, S., Levi Eshkol School of Agriculture, Hebrew University of Jerusalem, Rehovot, 7610001, Israel;

Bar-El, S., School of Nutritional Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot, 7610001, Israel;

Reifen, R., School of Nutritional Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot, 7610001, Israel;

Avneri, A., Levi Eshkol School of Agriculture, Hebrew University of Jerusalem, Rehovot, 7610001, Israel;

Facilitators :
From page:
3837
To page:
3841
(
Total pages:
5
)
Abstract:

The increasing demand for spelt products requires the baking industry to develop accurate and efficient tools to differentiate between spelt and bread wheat grains. We subjected a 272-sample spelt-bread wheat set to several potential diagnostic methods. DNA markers for γ-gliadin-D (GAG56D), γ-gliadin-B (GAG56B), and the Q-gene were used, alongside phenotypic assessment of ease-of-threshing and near-infrared spectroscopy (NIRS). The GAG56B and GAG56D markers demonstrated low diagnostic power in comparison to the Q-gene genotyping, which showed full accordance with the threshing phenotype, providing a highly accurate distinction between bread wheat and spelt kernels. A highly reliable Q classification was based on a three-waveband NIR model [Kappa (0.97), R-square (0.93)], which suggested that this gene influences grain characteristics. Our data ruled out a protein concentration bias of the NIRS-based diagnosis. These findings highlight the Q gene and NIRS as important, valuable, but simple tools for distinguishing between bread wheat and spelt. © 2019 American Chemical Society.

Note:
Related Files :
Adulteration
Food Products
Genes
Grain
Grain (agricultural product)
NIRS
wheat
עוד תגיות
תוכן קשור
More details
DOI :
10.1021/acs.jafc.9b00131
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
40039
Last updated date:
02/03/2022 17:27
Creation date:
15/04/2019 12:24
Scientific Publication
Distinguishing between Bread Wheat and Spelt Grains Using Molecular Markers and Spectroscopy
67

Abbo, S., Levi Eshkol School of Agriculture, Hebrew University of Jerusalem, Rehovot, 7610001, Israel;

Bar-El, S., School of Nutritional Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot, 7610001, Israel;

Reifen, R., School of Nutritional Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot, 7610001, Israel;

Avneri, A., Levi Eshkol School of Agriculture, Hebrew University of Jerusalem, Rehovot, 7610001, Israel;

Distinguishing between Bread Wheat and Spelt Grains Using Molecular Markers and Spectroscopy

The increasing demand for spelt products requires the baking industry to develop accurate and efficient tools to differentiate between spelt and bread wheat grains. We subjected a 272-sample spelt-bread wheat set to several potential diagnostic methods. DNA markers for γ-gliadin-D (GAG56D), γ-gliadin-B (GAG56B), and the Q-gene were used, alongside phenotypic assessment of ease-of-threshing and near-infrared spectroscopy (NIRS). The GAG56B and GAG56D markers demonstrated low diagnostic power in comparison to the Q-gene genotyping, which showed full accordance with the threshing phenotype, providing a highly accurate distinction between bread wheat and spelt kernels. A highly reliable Q classification was based on a three-waveband NIR model [Kappa (0.97), R-square (0.93)], which suggested that this gene influences grain characteristics. Our data ruled out a protein concentration bias of the NIRS-based diagnosis. These findings highlight the Q gene and NIRS as important, valuable, but simple tools for distinguishing between bread wheat and spelt. © 2019 American Chemical Society.

Scientific Publication
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