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Activation of simian virus 40 by transfer of isolated chromosomes from transformed cells
Year:
1974
Source of publication :
Virology
Authors :
Shani, Moshe
;
.
Volume :
61
Co-Authors:
Shani, M., Department of Genetics, Weizmann Institute of Science, Rehovot, Israel
Huberman, E., Department of Genetics, Weizmann Institute of Science, Rehovot, Israel
Aloni, Y., Department of Genetics, Weizmann Institute of Science, Rehovot, Israel
Sachs, L., Department of Genetics, Weizmann Institute of Science, Rehovot, Israel
Facilitators :
From page:
303
To page:
305
(
Total pages:
3
)
Abstract:
Metaphase chromosomes isolated from an inducible SV40 transformed Chinese hamster cell line with no detectable infectious virus or free virus DNA, were transferred into SV40 permissive BSC1 cells. This chromosome transfer resulted in the appearance of SV40 T-antigen in one per 104 treated cells and in the activation of infectious virus in one per 4 × 105 treated cells. No appearance of T-antigen or virus activation was obtained with sonicated chromosomes, whole nuclei, DNA, or lysates from the transformed cells. The results show the chromosomal location of the SV40 genome and the activation of viral genes after chromosome transfer. © 1974.
Note:
Related Files :
Animal
Antigens, Viral
cell nucleus
Chromosomes
Genes
microorganism
virus replication
Show More
Related Content
More details
DOI :
10.1016/0042-6822(74)90266-9
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
21597
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:45
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Scientific Publication
Activation of simian virus 40 by transfer of isolated chromosomes from transformed cells
61
Shani, M., Department of Genetics, Weizmann Institute of Science, Rehovot, Israel
Huberman, E., Department of Genetics, Weizmann Institute of Science, Rehovot, Israel
Aloni, Y., Department of Genetics, Weizmann Institute of Science, Rehovot, Israel
Sachs, L., Department of Genetics, Weizmann Institute of Science, Rehovot, Israel
Activation of simian virus 40 by transfer of isolated chromosomes from transformed cells
Metaphase chromosomes isolated from an inducible SV40 transformed Chinese hamster cell line with no detectable infectious virus or free virus DNA, were transferred into SV40 permissive BSC1 cells. This chromosome transfer resulted in the appearance of SV40 T-antigen in one per 104 treated cells and in the activation of infectious virus in one per 4 × 105 treated cells. No appearance of T-antigen or virus activation was obtained with sonicated chromosomes, whole nuclei, DNA, or lysates from the transformed cells. The results show the chromosomal location of the SV40 genome and the activation of viral genes after chromosome transfer. © 1974.
Scientific Publication
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