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פותח על ידי קלירמאש פתרונות בע"מ -
Gene activation by transfer of isolated mammalian chromosomes. II. Activation of simian virus 40 by transfer of fractionated chromosomes from transformed cells
Year:
1976
Source of publication :
Virology
Authors :
שני, משה
;
.
Volume :
70
Co-Authors:
Shani, M., Department of Genetics, Weizmann Institute of Science, Rehovot, Israel
Aloni, Y., Department of Genetics, Weizmann Institute of Science, Rehovot, Israel
Huberman, E., Department of Genetics, Weizmann Institute of Science, Rehovot, Israel
Sachs, L., Department of Genetics, Weizmann Institute of Science, Rehovot, Israel
Facilitators :
From page:
201
To page:
205
(
Total pages:
5
)
Abstract:
Metaphase chromosomes from an inducible SV40-transformed cell line with no detectable infectious virus or free virus DNA were fractionated into four fractions. The chromosomes were transferred into BSC-1 cells which are permissive for SV40-virus replication. The four chromosome fractions were analysed for their uptake by electron microscopy, for virus-DNA sequences by nucleic-acid hybridization, and for their activation of infectious virus. Chromatin material from all four fractions were observed in the cytoplasm of the cells after chromosome transfer. Virus-DNA sequences were detected by nucleic-acid hybridization in three of the four fractions, whereas infectious virus was activated after chromosome uptake of two of the fractions. The smallest chromosomes did not appear to contain either activatible SV40 genomes or integrated SV40-DNA sequences. It is suggested that transfer of fractionated chromosomes may also be useful for studies on the activation of other genes. © 1976.
Note:
Related Files :
Animal
Base Sequence
Cell Division
cell line
cell transformation
Chromosomes
microorganism
virus replication
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/0042-6822(76)90253-1
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
27795
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:34
Scientific Publication
Gene activation by transfer of isolated mammalian chromosomes. II. Activation of simian virus 40 by transfer of fractionated chromosomes from transformed cells
70
Shani, M., Department of Genetics, Weizmann Institute of Science, Rehovot, Israel
Aloni, Y., Department of Genetics, Weizmann Institute of Science, Rehovot, Israel
Huberman, E., Department of Genetics, Weizmann Institute of Science, Rehovot, Israel
Sachs, L., Department of Genetics, Weizmann Institute of Science, Rehovot, Israel
Gene activation by transfer of isolated mammalian chromosomes. II. Activation of simian virus 40 by transfer of fractionated chromosomes from transformed cells
Metaphase chromosomes from an inducible SV40-transformed cell line with no detectable infectious virus or free virus DNA were fractionated into four fractions. The chromosomes were transferred into BSC-1 cells which are permissive for SV40-virus replication. The four chromosome fractions were analysed for their uptake by electron microscopy, for virus-DNA sequences by nucleic-acid hybridization, and for their activation of infectious virus. Chromatin material from all four fractions were observed in the cytoplasm of the cells after chromosome transfer. Virus-DNA sequences were detected by nucleic-acid hybridization in three of the four fractions, whereas infectious virus was activated after chromosome uptake of two of the fractions. The smallest chromosomes did not appear to contain either activatible SV40 genomes or integrated SV40-DNA sequences. It is suggested that transfer of fractionated chromosomes may also be useful for studies on the activation of other genes. © 1976.
Scientific Publication
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