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Molecular analyses of Colletotrichum species from almond and other fruits
Year:
2000
Source of publication :
Phytopathology
Authors :
Freeman, Stanley
;
.
Maymon, Marcel
;
.
Minz, Dror
;
.
Shabi, Ezra
;
.
Volume :
90
Co-Authors:
Freeman, S., Department of Plant Pathology, ARO, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Minz, D., Department of Soil Sciences, ARO, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Jurkevitch, E., Dept. Plant Pathol. and Microbiol., Hebrew University of Jerusalem, Fac. Agric., Food Environ. Qual. S., Rehovot 76100, Israel
Maymon, M., Department of Plant Pathology, ARO, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Shabi, E., Department of Plant Pathology, ARO, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Facilitators :
From page:
608
To page:
614
(
Total pages:
7
)
Abstract:
Isolates of Colletotrichum spp. from almond, avocado, and strawberry from Israel and isolates of the pink subpopulation from almond from the United States were characterized by various molecular methods and compared with morphological identification. Taxon-specific primer analysis grouped the avocado isolates within the species C. gloeosporioides and the U.S. almond and Israeli strawberry isolates within the species C. acutatum. However, the Israeli almond isolates, previously identified morphologically as C. gloeosporioides, reacted with C. acutatum-specific primers. Arbitrarily primed polymerase chain reaction and A+T-rich DNA analyses determined that each population from almond and strawberry was distinct and clonal. Sequence analysis of the complete internal transcribed spacer (ITS) region (ITS 1-5.8S-ITS 2) revealed a similarity of between 97.03 and 98.72% among almond isolates from Israel, C. acutatum almond isolates from the United States, and C. acutatum strawberry isolates from Israel. Similarity of the above populations to that of C. gloeosporioides of avocado was between 92.42 and 92.86%. DNA sequence analysis of the entire ITS region supported the phylogeny inferred from the ITS 1 tree of 14 different Colletotrichum species. Although morphological criteria indicated that the Israeli isolates from almond are unique, this population was grouped within the C. acutatum species according to molecular analyses.
Note:
Related Files :
Avocado
Colletotrichum
Israel
Polymerase Chain Reaction
sequence analysis
United States
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More details
DOI :
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
22582
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:53
Scientific Publication
Molecular analyses of Colletotrichum species from almond and other fruits
90
Freeman, S., Department of Plant Pathology, ARO, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Minz, D., Department of Soil Sciences, ARO, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Jurkevitch, E., Dept. Plant Pathol. and Microbiol., Hebrew University of Jerusalem, Fac. Agric., Food Environ. Qual. S., Rehovot 76100, Israel
Maymon, M., Department of Plant Pathology, ARO, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Shabi, E., Department of Plant Pathology, ARO, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Molecular analyses of Colletotrichum species from almond and other fruits
Isolates of Colletotrichum spp. from almond, avocado, and strawberry from Israel and isolates of the pink subpopulation from almond from the United States were characterized by various molecular methods and compared with morphological identification. Taxon-specific primer analysis grouped the avocado isolates within the species C. gloeosporioides and the U.S. almond and Israeli strawberry isolates within the species C. acutatum. However, the Israeli almond isolates, previously identified morphologically as C. gloeosporioides, reacted with C. acutatum-specific primers. Arbitrarily primed polymerase chain reaction and A+T-rich DNA analyses determined that each population from almond and strawberry was distinct and clonal. Sequence analysis of the complete internal transcribed spacer (ITS) region (ITS 1-5.8S-ITS 2) revealed a similarity of between 97.03 and 98.72% among almond isolates from Israel, C. acutatum almond isolates from the United States, and C. acutatum strawberry isolates from Israel. Similarity of the above populations to that of C. gloeosporioides of avocado was between 92.42 and 92.86%. DNA sequence analysis of the entire ITS region supported the phylogeny inferred from the ITS 1 tree of 14 different Colletotrichum species. Although morphological criteria indicated that the Israeli isolates from almond are unique, this population was grouped within the C. acutatum species according to molecular analyses.
Scientific Publication
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