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An exercise in the formation of potato microtubers in tissue culture
Year:
1997
Source of publication :
Journal of Biological Education
Authors :
Steinitz, Benjamin
;
.
Volume :
31
Co-Authors:
Steinitz, B., Department of Plant Genetics, Institute of Field and Garden Crops, Agricultural Research Organization, POB 6, Bet Dagan 50250, Israel
Facilitators :
From page:
119
To page:
124
(
Total pages:
6
)
Abstract:
Regeneration of potato (Solanum tuberosum L.) microtubers in tissue culture is used as a model simulating the developmental physiology of underground storage organ formation. Two exercises are described: (i) induction of microtubers on tissue cultured derived single nodal explants, incubated on an agar-solidified Murashige and Skoog basal medium devoid of plant growth regulators, but supplemented with high sucrose concentration; and (ii) prevention of sucrose-mediated microtuberization by adding gibberellic acid (GA3) to the medium. The objectives of these experiments were to (a) illustrate that, from a developmental standpoint, underground storage organs are shortened, thickened stems; and (b) show that sucrose participates in tuber induction in a growth regulator-like mode of action.
Note:
Related Files :
plant tissue culture
Solanum tuberosum L
Teaching
Tuberization
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More details
DOI :
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
25772
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:17
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Scientific Publication
An exercise in the formation of potato microtubers in tissue culture
31
Steinitz, B., Department of Plant Genetics, Institute of Field and Garden Crops, Agricultural Research Organization, POB 6, Bet Dagan 50250, Israel
An exercise in the formation of potato microtubers in tissue culture
Regeneration of potato (Solanum tuberosum L.) microtubers in tissue culture is used as a model simulating the developmental physiology of underground storage organ formation. Two exercises are described: (i) induction of microtubers on tissue cultured derived single nodal explants, incubated on an agar-solidified Murashige and Skoog basal medium devoid of plant growth regulators, but supplemented with high sucrose concentration; and (ii) prevention of sucrose-mediated microtuberization by adding gibberellic acid (GA3) to the medium. The objectives of these experiments were to (a) illustrate that, from a developmental standpoint, underground storage organs are shortened, thickened stems; and (b) show that sucrose participates in tuber induction in a growth regulator-like mode of action.
Scientific Publication
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