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Cyclic Amp Response to Various Haemopoietic Regulators
Year:
1985
Source of publication :
Cell Proliferation
Authors :
Pines, Mark
;
.
Volume :
18
Co-Authors:
Wdzieczak‐Bakala, J., Unité de Cinétique Cellulaire Inserm U250, Institut Gustave-Roussy, Villejuif, 94805, France
Pines, M., The Volcani Center, Institute of Animal Science, P.O.B.6, Bet Dagan, Bet Dagan, 50-200, Israel
Guigon, M., Unité de Cinétique Cellulaire Inserm U250, Institut Gustave-Roussy, Villejuif, 94805, France
Lenfant, M., ICSN, CNRS, Gif-Sur-Yvette, 91190, France
Facilitators :
From page:
297
To page:
306
(
Total pages:
10
)
Abstract:
Diffusible inhibitors and stimulators are involved in the regulation of bone marrow pluripotent stem cell (CFU‐S) proliferation. We have previously shown the existence of CFU‐S inhibitors in foetal calf marrow and liver and have started their purification. the lack of a simple and time‐saving test to determine the kinetic state of CFU‐S and the activity of the inhibitors led us to explore the possibility of a biochemical proliferation marker that could be used for screening purpose. Since it was shown that cyclic AMP was implicated in the regulation of CFU‐S proliferation, it was of interest to study the variations in cAMP levels after stimulation and inhibition of CFU‐S entry into cycle. the results of in vitro experiments showed that the increase in cAMP levels observed in bone marrow cells after incubation with different haemopoietic stimulators was specific neither for bone marrow cells nor for the various haematopoietic regulators. In the in vivo experiments, an increased cAMP level was observed 8 hr after one injection of Ara‐C at the time when CFU‐S are recruited into S phase. However, no modification of cAMP levels has been observed after injection of CFU‐S inhibitors in the Ara C‐treated mice. Although cAMP does not seem to be a suitable marker for testing the activity of inhibitory fractions during the purification process, this work has contributed to the study of CFU‐S stimulators. Copyright © 1985, Wiley Blackwell. All rights reserved
Note:
Related Files :
blood and hemopoietic system
Cyclic AMP
Show More
Related Content
More details
DOI :
10.1111/j.1365-2184.1985.tb00659.x
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
30096
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:51
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Scientific Publication
Cyclic Amp Response to Various Haemopoietic Regulators
18
Wdzieczak‐Bakala, J., Unité de Cinétique Cellulaire Inserm U250, Institut Gustave-Roussy, Villejuif, 94805, France
Pines, M., The Volcani Center, Institute of Animal Science, P.O.B.6, Bet Dagan, Bet Dagan, 50-200, Israel
Guigon, M., Unité de Cinétique Cellulaire Inserm U250, Institut Gustave-Roussy, Villejuif, 94805, France
Lenfant, M., ICSN, CNRS, Gif-Sur-Yvette, 91190, France
Cyclic Amp Response to Various Haemopoietic Regulators
Diffusible inhibitors and stimulators are involved in the regulation of bone marrow pluripotent stem cell (CFU‐S) proliferation. We have previously shown the existence of CFU‐S inhibitors in foetal calf marrow and liver and have started their purification. the lack of a simple and time‐saving test to determine the kinetic state of CFU‐S and the activity of the inhibitors led us to explore the possibility of a biochemical proliferation marker that could be used for screening purpose. Since it was shown that cyclic AMP was implicated in the regulation of CFU‐S proliferation, it was of interest to study the variations in cAMP levels after stimulation and inhibition of CFU‐S entry into cycle. the results of in vitro experiments showed that the increase in cAMP levels observed in bone marrow cells after incubation with different haemopoietic stimulators was specific neither for bone marrow cells nor for the various haematopoietic regulators. In the in vivo experiments, an increased cAMP level was observed 8 hr after one injection of Ara‐C at the time when CFU‐S are recruited into S phase. However, no modification of cAMP levels has been observed after injection of CFU‐S inhibitors in the Ara C‐treated mice. Although cAMP does not seem to be a suitable marker for testing the activity of inhibitory fractions during the purification process, this work has contributed to the study of CFU‐S stimulators. Copyright © 1985, Wiley Blackwell. All rights reserved
Scientific Publication
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