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Sucrose Synthase and Fructokinase Are Required for Proper Meristematic and Vascular Development
Year:
2022
Source of publication :
Plants
Authors :
Belausov, Eduard
;
.
Egbaria, Aiman
;
.
Lugassi, Nitsan
;
.
Stein, Ofer
;
.
Zemach, Hanita
;
.
Volume :
Co-Authors:

Nitsan Lugassi
Ofer Stein
Aiman Egbaria
Eduard Belausov
Hanita Zemach
Tal Arad
David Granot
Nir Carmi 

Facilitators :
From page:
0
To page:
0
(
Total pages:
1
)
Abstract:

Sucrose synthase (SuSy) and fructokinase (FRK) work together to control carbohydrate flux in sink tissues. SuSy cleaves sucrose into fructose and UDP-glucose; whereas FRK phosphorylates fructose. Previous results have shown that suppression of the SUS1,3&4 genes by SUS-RNAi alters auxin transport in the shoot apical meristems of tomato plants and affects cotyledons and leaf structure; whereas antisense suppression of FRK2 affects vascular development. To explore the joint developmental roles of SuSy and FRK, we crossed SUS-RNAi plants with FRK2-antisense plants to create double-mutant plants. The double-mutant plants exhibited novel phenotypes that were absent from the parent lines. About a third of the plants showed arrested shoot apical meristem around the transition to flowering and developed ectopic meristems. Use of the auxin reporter DR5::VENUS revealed a significantly reduced auxin response in the shoot apical meristems of the double-mutant, indicating that auxin levels were low. Altered inflorescence phyllotaxis and significant disorientation of vascular tissues were also observed. In addition, the fruits and the seeds of the double-mutant plants were very small and the seeds had very low germination rates. These results show that SUS1,3&4 and FRK2 enzymes are jointly essential for proper meristematic and vascular development, and for fruit and seed development.

Note:
Related Files :
Auxin
phyllotaxis
Shoot apical meristem
Sugar metabolism
trichomes density
vascular tissues development
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Related Content
More details
DOI :
10.3390/plants11081035
Article number:
0
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
58734
Last updated date:
27/04/2022 16:01
Creation date:
26/04/2022 14:35
Scientific Publication
Sucrose Synthase and Fructokinase Are Required for Proper Meristematic and Vascular Development

Nitsan Lugassi
Ofer Stein
Aiman Egbaria
Eduard Belausov
Hanita Zemach
Tal Arad
David Granot
Nir Carmi 

Sucrose Synthase and Fructokinase Are Required for Proper Meristematic and Vascular Development

Sucrose synthase (SuSy) and fructokinase (FRK) work together to control carbohydrate flux in sink tissues. SuSy cleaves sucrose into fructose and UDP-glucose; whereas FRK phosphorylates fructose. Previous results have shown that suppression of the SUS1,3&4 genes by SUS-RNAi alters auxin transport in the shoot apical meristems of tomato plants and affects cotyledons and leaf structure; whereas antisense suppression of FRK2 affects vascular development. To explore the joint developmental roles of SuSy and FRK, we crossed SUS-RNAi plants with FRK2-antisense plants to create double-mutant plants. The double-mutant plants exhibited novel phenotypes that were absent from the parent lines. About a third of the plants showed arrested shoot apical meristem around the transition to flowering and developed ectopic meristems. Use of the auxin reporter DR5::VENUS revealed a significantly reduced auxin response in the shoot apical meristems of the double-mutant, indicating that auxin levels were low. Altered inflorescence phyllotaxis and significant disorientation of vascular tissues were also observed. In addition, the fruits and the seeds of the double-mutant plants were very small and the seeds had very low germination rates. These results show that SUS1,3&4 and FRK2 enzymes are jointly essential for proper meristematic and vascular development, and for fruit and seed development.

Scientific Publication
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