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פותח על ידי קלירמאש פתרונות בע"מ -
Grouping of potato isolates of PVY based on the 5'-UTR nucleotide sequence
Year:
2005
Source of publication :
Phytopathologia Mediterranea
Authors :
מסלנין, לודמילה
;
.
רוזנר, אריה
;
.
Volume :
44
Co-Authors:
Rosner, A., Virology Department, ARO, The Volcani Center, Bet Dagan 50250, Israel
Maslenin, L., Virology Department, ARO, The Volcani Center, Bet Dagan 50250, Israel
Facilitators :
From page:
12
To page:
16
(
Total pages:
5
)
Abstract:
Potato isolates of Potato virus Y (PVY) were divided into two major groups based on nucleotide sequence homology in the 5'-end region of their genomes. Several characteristic nucleotide modifications differentiated the two virus groups. Sequence similarity within members of each group was 89-100%, whereas between members of the respective groups there was sequence divergence of up to 33%. The two groups were distinguished by restriction cleavage of the virus PCR products elicited by group-specific restriction endonucleases: Xho II and Nsp I (group I) and Mbo II, Fok I and Nco I (group II). The sites of the first three enzymes (group I-specific) were sequentially positioned so that a sequence of 16 nucleotides preceding the ATG codon could be determined in this way. Group I included members of the tobacco necrotic (N) and non-necrotic (O) strains of PVY, whereas the potato tuber necrotic isolate NTN-H belonged to the second group, which included also non-tuber and tobacco leaf necrotic members. It seems therefore that the biological characteristics of the tuber or tobacco leaf necrosis are not related to nucleotide modifications at the 5'-UTR but rather to group-specific differences.
Note:
Related Files :
Nicotiana tabacum
PCR
Potato virus Y
Potyvirus
PTNRD
RFLP
Solanum tuberosum
Strain differentiation
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
20506
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2018 23:37
Scientific Publication
Grouping of potato isolates of PVY based on the 5'-UTR nucleotide sequence
44
Rosner, A., Virology Department, ARO, The Volcani Center, Bet Dagan 50250, Israel
Maslenin, L., Virology Department, ARO, The Volcani Center, Bet Dagan 50250, Israel
Grouping of potato isolates of PVY based on the 5'-UTR nucleotide sequence
Potato isolates of Potato virus Y (PVY) were divided into two major groups based on nucleotide sequence homology in the 5'-end region of their genomes. Several characteristic nucleotide modifications differentiated the two virus groups. Sequence similarity within members of each group was 89-100%, whereas between members of the respective groups there was sequence divergence of up to 33%. The two groups were distinguished by restriction cleavage of the virus PCR products elicited by group-specific restriction endonucleases: Xho II and Nsp I (group I) and Mbo II, Fok I and Nco I (group II). The sites of the first three enzymes (group I-specific) were sequentially positioned so that a sequence of 16 nucleotides preceding the ATG codon could be determined in this way. Group I included members of the tobacco necrotic (N) and non-necrotic (O) strains of PVY, whereas the potato tuber necrotic isolate NTN-H belonged to the second group, which included also non-tuber and tobacco leaf necrotic members. It seems therefore that the biological characteristics of the tuber or tobacco leaf necrosis are not related to nucleotide modifications at the 5'-UTR but rather to group-specific differences.
Scientific Publication
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