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פותח על ידי קלירמאש פתרונות בע"מ -
Molecular characterisation and differential diagnosis of a necrotic PVY isolate in tomato
Year:
2000
Source of publication :
Annals of Applied Biology
Authors :
אנטיגנוס, יחזקאל
;
.
לכמן, עודד
;
.
מסלנין, לודמילה
;
.
פרלסמן, מלניה
;
.
רוזנר, אריה
;
.
Volume :
137
Co-Authors:
Rosner, A., Virology Department, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Lachman, A., Virology Department, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Pearlsman, M., Virology Department, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Maslenin, L., Virology Department, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Antignus, Y., Virology Department, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Facilitators :
From page:
253
To page:
257
(
Total pages:
5
)
Abstract:
A PVY isolate which causes strong necrotic lesions in tomato was isolated and characterised. It did not infect potato and did not react with antiserum specific to the N-strain of PVY. Restriction endonuclease cleavage patterns of PCR products derived from the 5′-end of the virus genome (Hinc II and Rsa I) clearly distinguished the tomato, pepper and potato strains. Additional sequence analysis indicated that the tomato and pepper isolates were closely related, while both markedly differed from the necrotic strains of potato. Hence, it was concluded that the necrotic tomato isolate is uniquely specific for tomato.
Note:
Related Files :
Cell Division
Polymerase Chain Reaction
restriction endonuclease
sequence analysis
Solanum tuberosum
Strain differentiation
virus genome
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
26265
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:21
Scientific Publication
Molecular characterisation and differential diagnosis of a necrotic PVY isolate in tomato
137
Rosner, A., Virology Department, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Lachman, A., Virology Department, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Pearlsman, M., Virology Department, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Maslenin, L., Virology Department, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Antignus, Y., Virology Department, Agricultural Research Organization, Volcani Center, Bet Dagan 50250, Israel
Molecular characterisation and differential diagnosis of a necrotic PVY isolate in tomato
A PVY isolate which causes strong necrotic lesions in tomato was isolated and characterised. It did not infect potato and did not react with antiserum specific to the N-strain of PVY. Restriction endonuclease cleavage patterns of PCR products derived from the 5′-end of the virus genome (Hinc II and Rsa I) clearly distinguished the tomato, pepper and potato strains. Additional sequence analysis indicated that the tomato and pepper isolates were closely related, while both markedly differed from the necrotic strains of potato. Hence, it was concluded that the necrotic tomato isolate is uniquely specific for tomato.
Scientific Publication
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