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Differential activation of ammonium transporters during the accumulation of ammonia by Colletotrichum gloeosporioides and its effect on appressoria formation and pathogenicity
Year:
2013
Authors :
מיארה, איתי
;
.
פרוסקי, דב
;
.
קובילר, אילנה
;
.
שרמן, עמיר
;
.
Volume :
26
Co-Authors:
Shnaiderman, C., Department of Postharvest Science of Fresh Produce, ARO, Bet Dagan, Israel, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot, Israel
Miyara, I., Department of Postharvest Science of Fresh Produce, ARO, Bet Dagan, Israel
Kobiler, I., Department of Postharvest Science of Fresh Produce, ARO, Bet Dagan, Israel
Sherman, A., Genomic Unit, Plant Sciences Institute, ARO, Bet Dagan, Israel
Prusky, D., Department of Postharvest Science of Fresh Produce, ARO, Bet Dagan, Israel
Facilitators :
From page:
345
To page:
355
(
Total pages:
11
)
Abstract:
Ammonium secreted by the post-harvest pathogen Colletotrichum gloeosporioides during host colonization accumulates in the host environment due to enhanced fungal nitrogen metabolism. Two types of ammonium transporterencoding genes, AMET and MEP, are expressed during pathogenicity. Gene disruption of AMET, a gene modulating ammonia secretion, showed twofold reduced ammonia secretion and 45% less colonization on avocado fruit, suggesting a contribution to pathogenicity. MEPB, a gene modulating ammonium transport, is expressed by C. gloeosporioides during pathogenicity and starvation conditions in culture. Gene disruption of MEPB, the most highly expressed gene of the MEP family, resulted in twofold overexpression of MEPA and MEPC but reduced colonization, suggesting MEPB expression's contribution to pathogenicity. Analysis of internal and external ammonia accumulation by Δmep B strains in mycelia and germinated spores showed rapid uptake and accumulation, and reduced secretion of ammonia in the mutant versus wild-type (WT) strains. Ammonia uptake by the WT germinating spores but not by the Δmep B strain with compromised ammonium transport activated cAMP and transcription of PKA subunits PKAR and PKA2. Δmep B mutants showed 75% less appressorium formation and colonization than the WT, which was partially restored by 10 mM exogenous ammonia. Thus, whereas both AMET and MEPB genes modulate ammonia secretion, only MEPB contributes to ammonia accumulation by mycelia and germinating spores that activate the cAMP pathways, inducing the morphogenetic processes contributing to C. gloeosporioides pathogenicity. © 2013 The American Phytopathological Society.
Note:
Related Files :
Colletotrichum
Cyclic AMP
Genetics
Growth, Development and Aging
metabolism
Microbiology
mycelium
phenotype
Plant Disease
עוד תגיות
תוכן קשור
More details
DOI :
10.1094/MPMI-07-12-0170-R
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
25006
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:11
You may also be interested in
Scientific Publication
Differential activation of ammonium transporters during the accumulation of ammonia by Colletotrichum gloeosporioides and its effect on appressoria formation and pathogenicity
26
Shnaiderman, C., Department of Postharvest Science of Fresh Produce, ARO, Bet Dagan, Israel, Robert H. Smith Faculty of Agriculture, Food and Environment, Hebrew University of Jerusalem, Rehovot, Israel
Miyara, I., Department of Postharvest Science of Fresh Produce, ARO, Bet Dagan, Israel
Kobiler, I., Department of Postharvest Science of Fresh Produce, ARO, Bet Dagan, Israel
Sherman, A., Genomic Unit, Plant Sciences Institute, ARO, Bet Dagan, Israel
Prusky, D., Department of Postharvest Science of Fresh Produce, ARO, Bet Dagan, Israel
Differential activation of ammonium transporters during the accumulation of ammonia by Colletotrichum gloeosporioides and its effect on appressoria formation and pathogenicity
Ammonium secreted by the post-harvest pathogen Colletotrichum gloeosporioides during host colonization accumulates in the host environment due to enhanced fungal nitrogen metabolism. Two types of ammonium transporterencoding genes, AMET and MEP, are expressed during pathogenicity. Gene disruption of AMET, a gene modulating ammonia secretion, showed twofold reduced ammonia secretion and 45% less colonization on avocado fruit, suggesting a contribution to pathogenicity. MEPB, a gene modulating ammonium transport, is expressed by C. gloeosporioides during pathogenicity and starvation conditions in culture. Gene disruption of MEPB, the most highly expressed gene of the MEP family, resulted in twofold overexpression of MEPA and MEPC but reduced colonization, suggesting MEPB expression's contribution to pathogenicity. Analysis of internal and external ammonia accumulation by Δmep B strains in mycelia and germinated spores showed rapid uptake and accumulation, and reduced secretion of ammonia in the mutant versus wild-type (WT) strains. Ammonia uptake by the WT germinating spores but not by the Δmep B strain with compromised ammonium transport activated cAMP and transcription of PKA subunits PKAR and PKA2. Δmep B mutants showed 75% less appressorium formation and colonization than the WT, which was partially restored by 10 mM exogenous ammonia. Thus, whereas both AMET and MEPB genes modulate ammonia secretion, only MEPB contributes to ammonia accumulation by mycelia and germinating spores that activate the cAMP pathways, inducing the morphogenetic processes contributing to C. gloeosporioides pathogenicity. © 2013 The American Phytopathological Society.
Scientific Publication
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