Muruganantham, M., Department of Biotechnology, Bharathidasan University, Tiruchirappalli 620 024, India
Ganapathi, A., Department of Biotechnology, Bharathidasan University, Tiruchirappalli 620 024, India
Amutha, S., Department of Biotechnology, Bharathidasan University, Tiruchirappalli 620 024, India
Vengadesan, G., Department of Biotechnology, Bharathidasan University, Tiruchirappalli 620 024, India
Selvaraj, N., Department of Botany, Periyar EVR College (Autonomous), Tiruchirappalli 620 023, India

Eighteen-day-old immature cotyledonary node explants (18 d after anthesis) of black gram produced multiple shoots in MS salts+B5 vitamins containing medium in the presence of BA (1.0 mg/L), TDZ (0.1 mg/L) and AdS (15 mg/L). Maximum shoot proliferation (28 shoot/explant) occurred at the end of second subculture after 45 d. Periodic excision of regenerated shoots from explants increased shoot regeneration efficiency during subculture. The combination of TDZ and AdS with BA significantly increased shoot proliferation. Elongation and rooting were performed in GA3 (0.6 mg/L) and IBA (1.0 mg/L) containing media, respectively. The in vitro raised plantlets were acclimatized in green house and successfully transplanted to the field with a survival rate of 60%.