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פותח על ידי קלירמאש פתרונות בע"מ -
Serological characterisation of citrus tristeza virus isolates from Israel
Year:
1994
Source of publication :
Annals of Applied Biology
Authors :
בר-יוסף, משה
;
.
גפני, רון
;
.
מוואסי, מוניר
;
.
שליטין, ד'
;
.
Volume :
125
Co-Authors:
SHALITIN, D., S Tolkowsky Laboratory, Agricultural Research Organisation, The Volcani Center, Bet Dagan, Israel
MAWASSI, M., S Tolkowsky Laboratory, Agricultural Research Organisation, The Volcani Center, Bet Dagan, Israel
GAFNY, R., S Tolkowsky Laboratory, Agricultural Research Organisation, The Volcani Center, Bet Dagan, Israel
LEITNER, O., Department of Biological Sciences, Weizman Institute of Science, Rehovot, Israel
CABILLY, S., Department of Membrane and Biophysics, Weizman Institute of Science, Rehovot, Israel
ESHHAR, Z., Department of Chemical Immunology, Weizman Institute of Science, Rehovot, Israel
BAR‐JOSEPH, M., S Tolkowsky Laboratory, Agricultural Research Organisation, The Volcani Center, Bet Dagan, Israel
Facilitators :
From page:
105
To page:
113
(
Total pages:
9
)
Abstract:
Seven monoclonal antibodies (MAbs) showing homologous reactions with the VT strain of citrus tristeza virus (CTV) were tested against 21 CTV strains or isolates, representing the range of biological diversity and the geographical distribution of CTV in Israel. All the CTV strains gave positive reactions in ELISA with polyclonal antibodies and with one MAb (#25#2). Two MAbs; #13#21 and #3/7 reacted with 19 and 12 out of the 21 CTV strains, respectively. Seventeen CTV strains, including all those previously assigned by sequencing their coat protein gene (CPG) to the CPG‐VT group (Mawassi, Gafny & Bar‐Joseph, 1993), reacted with five or more MAbs. Four CTV strains of minor epidemiological importance, including two members of the CPG‐MT group, did not react with five or more of the MAbs. These results indicated the existence of two serogroups of Israeli CTV strains that can be differentiated by MAbs and which closely correlate with and extend the previous CPG grouping. The extensive biological variation within each CPG group confirms recent analyses suggesting that the CTV pathogenic traits are not necessarily associated with a sequence or antigenic variation of the CTV‐CPG. Copyright © 1994, Wiley Blackwell. All rights reserved
Note:
Related Files :
aphid transmissibility
coat protein gene
Monoclonal antibodies
pathogenic isolates
Tristeza‐epidemiology
עוד תגיות
תוכן קשור
More details
DOI :
10.1111/j.1744-7348.1994.tb04951.x
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
31710
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:04
You may also be interested in
Scientific Publication
Serological characterisation of citrus tristeza virus isolates from Israel
125
SHALITIN, D., S Tolkowsky Laboratory, Agricultural Research Organisation, The Volcani Center, Bet Dagan, Israel
MAWASSI, M., S Tolkowsky Laboratory, Agricultural Research Organisation, The Volcani Center, Bet Dagan, Israel
GAFNY, R., S Tolkowsky Laboratory, Agricultural Research Organisation, The Volcani Center, Bet Dagan, Israel
LEITNER, O., Department of Biological Sciences, Weizman Institute of Science, Rehovot, Israel
CABILLY, S., Department of Membrane and Biophysics, Weizman Institute of Science, Rehovot, Israel
ESHHAR, Z., Department of Chemical Immunology, Weizman Institute of Science, Rehovot, Israel
BAR‐JOSEPH, M., S Tolkowsky Laboratory, Agricultural Research Organisation, The Volcani Center, Bet Dagan, Israel
Serological characterisation of citrus tristeza virus isolates from Israel
Seven monoclonal antibodies (MAbs) showing homologous reactions with the VT strain of citrus tristeza virus (CTV) were tested against 21 CTV strains or isolates, representing the range of biological diversity and the geographical distribution of CTV in Israel. All the CTV strains gave positive reactions in ELISA with polyclonal antibodies and with one MAb (#25#2). Two MAbs; #13#21 and #3/7 reacted with 19 and 12 out of the 21 CTV strains, respectively. Seventeen CTV strains, including all those previously assigned by sequencing their coat protein gene (CPG) to the CPG‐VT group (Mawassi, Gafny & Bar‐Joseph, 1993), reacted with five or more MAbs. Four CTV strains of minor epidemiological importance, including two members of the CPG‐MT group, did not react with five or more of the MAbs. These results indicated the existence of two serogroups of Israeli CTV strains that can be differentiated by MAbs and which closely correlate with and extend the previous CPG grouping. The extensive biological variation within each CPG group confirms recent analyses suggesting that the CTV pathogenic traits are not necessarily associated with a sequence or antigenic variation of the CTV‐CPG. Copyright © 1994, Wiley Blackwell. All rights reserved
Scientific Publication
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