נגישות
menu      
חיפוש מתקדם
תחביר
חפש...
הספר "אוצר וולקני"
אודות
תנאי שימוש
ניהול
קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Characterization of the phenotypic and genotypic tolerance to abiotic stresses of natural populations of Heterorhabditis bacteriophora
Year:
2020
Source of publication :
Scientific Reports
Authors :
גלזר, איתמר
;
.
דורון-פייגנבאום, עדי
;
.
מנט, דנה
;
.
סלמה, ליאורה
;
.
Volume :
10
Co-Authors:

Levy, N. - Department of Entomology and the Nematology and Chemistry units, Plant Protection Institute; Agricultural Research Organization (ARO), the Volcani Center, Rishon Le Zion, Israel; The Robert H. Smith Faculty of Agriculture, Food & Environment the Hebrew University of Jerusalem, Rehovot, Israel.
Molina, C. - e-nema GmbH, Gesellschaft für Biotechnologie und biologischen Pflanzenschutz Klausdorfer Str. 28–36, Schwentinental, 24223, Germany.
Ehlers, R.-U. - e-nema GmbH, Gesellschaft für Biotechnologie und biologischen Pflanzenschutz Klausdorfer Str. 28–36, Schwentinental, 24223, Germany.

Facilitators :
From page:
0
To page:
0
(
Total pages:
1
)
Abstract:

Entomopathogenic nematodes are effective biocontrol agents against arthropod pests. However, their efficacy is limited due to sensitivity to environmental extremes. The objective of the present study was to establish a foundation of genetic-based selection tools for beneficial traits of heat and desiccation tolerance in entomopathogenic nematodes. Screening of natural populations enabled us to create a diverse genetic and phenotypic pool. Gene expression patterns and genomic variation were studied in natural isolates. Heterorhabditis isolates were phenotyped by heat- and desiccation-stress bioassays to determine their survival rates compared to a commercial line. Transcriptomic study was carried out for the commercial line, a high heat-tolerant strain, and for the natural, low heat-tolerant isolate. The results revealed a higher number of upregulated vs. downregulated transcripts in both isolates vs. their respective controls. Functional annotation of the differentially expressed transcripts revealed several known stress-related genes and pathways uniquely expressed. Genome sequencing of isolates with varied degrees of stress tolerance indicated variation among the isolates regardless of their phenotypic characterization. The obtained data lays the groundwork for future studies aimed at identifying genes and molecular markers as genetic selection tools for enhancement of entomopathogenic nematodes ability to withstand environmental stress conditions.

Note:
Related Files :
abiotic stress
Bioassay
drought
Environmental stress
natural population
survival rate
transcription initiation
עוד תגיות
תוכן קשור
More details
DOI :
10.1038/s41598-020-67097-0
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
48633
Last updated date:
02/03/2022 17:27
Creation date:
05/07/2020 16:35
You may also be interested in
Scientific Publication
Characterization of the phenotypic and genotypic tolerance to abiotic stresses of natural populations of Heterorhabditis bacteriophora
10

Levy, N. - Department of Entomology and the Nematology and Chemistry units, Plant Protection Institute; Agricultural Research Organization (ARO), the Volcani Center, Rishon Le Zion, Israel; The Robert H. Smith Faculty of Agriculture, Food & Environment the Hebrew University of Jerusalem, Rehovot, Israel.
Molina, C. - e-nema GmbH, Gesellschaft für Biotechnologie und biologischen Pflanzenschutz Klausdorfer Str. 28–36, Schwentinental, 24223, Germany.
Ehlers, R.-U. - e-nema GmbH, Gesellschaft für Biotechnologie und biologischen Pflanzenschutz Klausdorfer Str. 28–36, Schwentinental, 24223, Germany.

Characterization of the phenotypic and genotypic tolerance to abiotic stresses of natural populations of Heterorhabditis bacteriophora

Entomopathogenic nematodes are effective biocontrol agents against arthropod pests. However, their efficacy is limited due to sensitivity to environmental extremes. The objective of the present study was to establish a foundation of genetic-based selection tools for beneficial traits of heat and desiccation tolerance in entomopathogenic nematodes. Screening of natural populations enabled us to create a diverse genetic and phenotypic pool. Gene expression patterns and genomic variation were studied in natural isolates. Heterorhabditis isolates were phenotyped by heat- and desiccation-stress bioassays to determine their survival rates compared to a commercial line. Transcriptomic study was carried out for the commercial line, a high heat-tolerant strain, and for the natural, low heat-tolerant isolate. The results revealed a higher number of upregulated vs. downregulated transcripts in both isolates vs. their respective controls. Functional annotation of the differentially expressed transcripts revealed several known stress-related genes and pathways uniquely expressed. Genome sequencing of isolates with varied degrees of stress tolerance indicated variation among the isolates regardless of their phenotypic characterization. The obtained data lays the groundwork for future studies aimed at identifying genes and molecular markers as genetic selection tools for enhancement of entomopathogenic nematodes ability to withstand environmental stress conditions.

Scientific Publication
You may also be interested in