נגישות
menu      
Advanced Search
Syntax
Search...
Volcani treasures
About
Terms of use
Manage
Community:
אסיף מאגר המחקר החקלאי
Powered by ClearMash Solutions Ltd -
Control of cell-type specific gene expression in Dictyostelium by the general transcription factor GBF
Year:
1997
Source of publication :
Development
Authors :
Gollop, Rachel
;
.
Volume :
124
Co-Authors:
Gollop, R., Lab. of Cell./Developmental Biology, NIDDK (Bldg. 6/B1-22), National Institutes of Health, Bethesda, MD 20892, United States, Department of Fruit Tree Breeding, Institute of Horticulture, Volcani Center, PO Box 6, Bet-Dagan 50250, Israel
Kimmel, A.R., Lab. of Cell./Developmental Biology, NIDDK (Bldg. 6/B1-22), National Institutes of Health, Bethesda, MD 20892, United States
Facilitators :
From page:
3395
To page:
3405
(
Total pages:
11
)
Abstract:
To understand how positional information within an organism specifies patterning during development, we are analyzing spatially regulated gene expression in Dictyostelium. CAR3 is a member of the cAMP, 7-span receptor family which directs the transition from unicellular to multicellular organism and regulates cellular differentiation and pattern formation. CAR3 mRNA is expressed maximally at 8-10 hours of development, as individual cells aggregate and differentiate, and is accumulated to equivalent levels in all cells. CAR3 is also induced in shaking cultures by response to extracellular cAMP. We now show, by extensive mutagenesis, that the maximum length of contiguous sequences required for accurate spatiotemporal regulation of CAR3 is approx. 350 bp. These sequences include three significant elements located in upstream and transcribed regions. Arrays of G-boxes (GBF regulatory sites) are centered near positions -165 and +50 and, although either is sufficient for induction by cAMP and expression in prespore cells, both are required for expression in prestalk cells, Another GC-rich element near position -80 is required for maximal expression of prespore-specific constructs, although full-length promoters carrying clustered mutations through the -80 region are still expressed in all cells, but with slightly reduced expression, Spatiotemporal expression of CAR3 during development, thus, requires cell-specific combinatorial interactions of multiple but redundant regulatory components. These essential elements are located in upstream and transcribed regions. However, most surprisingly, a primary control for spatial patterning of CAR3 expression appears to be mediated by GBF, a general transcription factor expressed ubiquitously during Dictyostelium development following early aggregation.
Note:
Related Files :
Animals
Cyclic AMP
Development
DNA responsive element
gene expression
Genes, Fungal
mutation
Receptors
Show More
Related Content
More details
DOI :
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
24934
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:11
You may also be interested in
Scientific Publication
Control of cell-type specific gene expression in Dictyostelium by the general transcription factor GBF
124
Gollop, R., Lab. of Cell./Developmental Biology, NIDDK (Bldg. 6/B1-22), National Institutes of Health, Bethesda, MD 20892, United States, Department of Fruit Tree Breeding, Institute of Horticulture, Volcani Center, PO Box 6, Bet-Dagan 50250, Israel
Kimmel, A.R., Lab. of Cell./Developmental Biology, NIDDK (Bldg. 6/B1-22), National Institutes of Health, Bethesda, MD 20892, United States
Control of cell-type specific gene expression in Dictyostelium by the general transcription factor GBF
To understand how positional information within an organism specifies patterning during development, we are analyzing spatially regulated gene expression in Dictyostelium. CAR3 is a member of the cAMP, 7-span receptor family which directs the transition from unicellular to multicellular organism and regulates cellular differentiation and pattern formation. CAR3 mRNA is expressed maximally at 8-10 hours of development, as individual cells aggregate and differentiate, and is accumulated to equivalent levels in all cells. CAR3 is also induced in shaking cultures by response to extracellular cAMP. We now show, by extensive mutagenesis, that the maximum length of contiguous sequences required for accurate spatiotemporal regulation of CAR3 is approx. 350 bp. These sequences include three significant elements located in upstream and transcribed regions. Arrays of G-boxes (GBF regulatory sites) are centered near positions -165 and +50 and, although either is sufficient for induction by cAMP and expression in prespore cells, both are required for expression in prestalk cells, Another GC-rich element near position -80 is required for maximal expression of prespore-specific constructs, although full-length promoters carrying clustered mutations through the -80 region are still expressed in all cells, but with slightly reduced expression, Spatiotemporal expression of CAR3 during development, thus, requires cell-specific combinatorial interactions of multiple but redundant regulatory components. These essential elements are located in upstream and transcribed regions. However, most surprisingly, a primary control for spatial patterning of CAR3 expression appears to be mediated by GBF, a general transcription factor expressed ubiquitously during Dictyostelium development following early aggregation.
Scientific Publication
You may also be interested in