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The expression of estrogen receptor and estrogen effect in MBA-15 marrow stromal osteoblasts
Year:
1996
Source of publication :
Cell Biology International
Authors :
Knopov, Viktor
;
.
Shamay, Avi
;
.
Volume :
20
Co-Authors:
Shamay, A., Institute of Animal Science, ARO, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Knopov, V., Institute of Animal Science, ARO, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Benayahu, D., Dept. of Cell Biology and Histology, Sackler School of Medicine, Tel-Aviv University, Tel Aviv 66978, Israel
Facilitators :
From page:
401
To page:
405
(
Total pages:
5
)
Abstract:
MBA-15, a marrow stromal-derived cell line, was shown to express an estrogen receptor. This finding was confirmed by in situ hybridization and receptor binding assay. An exposure to estrogen (10-12-10-6M) in a dose response manner resulted in a decrease of cell proliferation as measured by MTT assay. Cell function was measured by enzymatic activities of two osteoblastic markers, CD1O/NEP and alkaline phosphatase. These enzymatic activities were elevated following the estrogen treatment. This model enabled direct evaluation of the estrogen effect on stroma osteoblast cells.
Note:
Related Files :
animal cell
Animals
bone marrow
Cell Division
Cell Proliferation
enzyme activity
mice
nitroblue tetrazolium test
osteoblast
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Related Content
More details
DOI :
10.1006/cbir.1996.0050
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
28681
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 00:41
Scientific Publication
The expression of estrogen receptor and estrogen effect in MBA-15 marrow stromal osteoblasts
20
Shamay, A., Institute of Animal Science, ARO, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Knopov, V., Institute of Animal Science, ARO, Volcani Center, P.O. Box 6, Bet Dagan 50250, Israel
Benayahu, D., Dept. of Cell Biology and Histology, Sackler School of Medicine, Tel-Aviv University, Tel Aviv 66978, Israel
The expression of estrogen receptor and estrogen effect in MBA-15 marrow stromal osteoblasts
MBA-15, a marrow stromal-derived cell line, was shown to express an estrogen receptor. This finding was confirmed by in situ hybridization and receptor binding assay. An exposure to estrogen (10-12-10-6M) in a dose response manner resulted in a decrease of cell proliferation as measured by MTT assay. Cell function was measured by enzymatic activities of two osteoblastic markers, CD1O/NEP and alkaline phosphatase. These enzymatic activities were elevated following the estrogen treatment. This model enabled direct evaluation of the estrogen effect on stroma osteoblast cells.
Scientific Publication
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