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Neuroprotective Effects of BrowZine Pulicaria incisa Infusion on Human Neuroblastoma Cells and Hippocampal Neurons
Year:
2022
Source of publication :
antioxidant
Authors :
Belausov, Eduard
;
.
Elmann, Anat
;
.
Tietel, Zipora
;
.
Volume :
Co-Authors:

Talya Barak 
Oshrat Miller
Sarit Melamed
Zipora Tietel 
Moti Harari 
Eduard Belausov
Anat Elmann

Facilitators :
From page:
0
To page:
0
(
Total pages:
1
)
Abstract:

Reactive oxygen species (ROS) and oxidative stress increase susceptibility to neurodegeneration and other age-related pathologies. We have previously demonstrated that an infusion prepared from Pulicaria incisa (Pi) has protective, anti-inflammatory, and antioxidative effects in glial cells. However, the neuroprotective activities of Pi infusion in cultured neurons and aging mice have never been studied. In the following study, the effects of Pi infusion were explored in a hydrogen peroxide (H2O2)-induced oxidative stress model in SH-SY5Y human neuroblastoma cells. Profiling of the infusion by gas chromatography-mass spectrometry identified chlorogenic acid, quercetin, and aucubin as some of its main constituents. H2O2-induced ROS accumulation and caspase 3 activity decreased SH-SY5Y viability and were prevented upon the pretreatment of cells with Pi infusion. Additionally, the Pi infusion upregulated cellular levels and the nuclear translocation of nuclear factor erythroid 2-related factor 2 (Nrf2) as well as the phosphorylation of cyclic AMP response element-binding protein (CREB). Aging mice treated daily for 18 months with Pi infusion exhibited reduced neuronal cell death in the hippocampus as compared to age-matched controls. We, therefore, propose Pi infusion as a candidate regulator of oxidative stress in the brain.

Note:
Related Files :
aging
CREB
hippocampus
neuroprotection
Nrf2
phytochemicals
Pulicaria incisa
reactive oxygen species
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More details
DOI :
10.3390/antiox12010032
Article number:
0
Affiliations:
Database:
PubMed
Publication Type:
article
;
.
Language:
Editors' remarks:
ID:
63298
Last updated date:
23/01/2023 17:28
Creation date:
23/01/2023 17:28
Scientific Publication
Neuroprotective Effects of BrowZine Pulicaria incisa Infusion on Human Neuroblastoma Cells and Hippocampal Neurons

Talya Barak 
Oshrat Miller
Sarit Melamed
Zipora Tietel 
Moti Harari 
Eduard Belausov
Anat Elmann

Neuroprotective Effects of BrowZine Pulicaria incisa Infusion on Human Neuroblastoma Cells and Hippocampal Neurons

Reactive oxygen species (ROS) and oxidative stress increase susceptibility to neurodegeneration and other age-related pathologies. We have previously demonstrated that an infusion prepared from Pulicaria incisa (Pi) has protective, anti-inflammatory, and antioxidative effects in glial cells. However, the neuroprotective activities of Pi infusion in cultured neurons and aging mice have never been studied. In the following study, the effects of Pi infusion were explored in a hydrogen peroxide (H2O2)-induced oxidative stress model in SH-SY5Y human neuroblastoma cells. Profiling of the infusion by gas chromatography-mass spectrometry identified chlorogenic acid, quercetin, and aucubin as some of its main constituents. H2O2-induced ROS accumulation and caspase 3 activity decreased SH-SY5Y viability and were prevented upon the pretreatment of cells with Pi infusion. Additionally, the Pi infusion upregulated cellular levels and the nuclear translocation of nuclear factor erythroid 2-related factor 2 (Nrf2) as well as the phosphorylation of cyclic AMP response element-binding protein (CREB). Aging mice treated daily for 18 months with Pi infusion exhibited reduced neuronal cell death in the hippocampus as compared to age-matched controls. We, therefore, propose Pi infusion as a candidate regulator of oxidative stress in the brain.

Scientific Publication
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