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Sol-gel entrapped levonorgestrel antibodies: Activity and structural changes as a function of different polymer formats
Year:
2010
Source of publication :
Materials
Authors :
Altstein, Miriam
;
.
Shalev, Moran
;
.
Volume :
4
Co-Authors:
Shale, M., Department of Entomology, Institute of Plant Protection, The Volcani Center, Bet Dagan 50250, Israel
Miriam, A., Department of Entomology, Institute of Plant Protection, The Volcani Center, Bet Dagan 50250, Israel
Facilitators :
From page:
469
To page:
486
(
Total pages:
18
)
Abstract:
The paper describes development of a sol-gel based immunoaffinity method for the steroid hormone levonorgestrel (LNG) and the effects of changes in the sol-gel matrix format on the activity of the entrapped antibodies (Abs) and on matrix structure. The best sol-gel format for Ab entrapment was found to be a tetramethoxysilane (TMOS) based matrix at a TMOS:water ratio of 1:8, containing 10% polyethylene glycol (PEG) of MW 0.4 kDa. Addition of higher percentages of PEG or a higher MW PEG did not improve activity. No activity was obtained with a TMOS:water ratio of 1:12, most likely because of the very dense polymer that resulted from these polymerization conditions. Only minor differences in the non-specific binding were obtained with the various formats. TMOS was found to be more effective than tetrakis (2-hydroxyethyl)orthosilicate (THEOS) for entrapment of anti-levonorgestrel (LNG) Abs. However, aging the THEOS-based sol-gel for a few weeks at 4 °C stabilized the entrapped Abs and increased its binding capacity. Confocal fluorescent microscopy with fluorescein isothiocyanate (FITC) labeled immunoglobulines (IgGs) entrapped in the sol-gel matrix showed that the entrapped Abs were distributed homogenously within the gel. Scanning electron microscopy (SEM) images have shown the diverse structures of the various sol-gel formats and precursors. © 2011 by the authors; licensee MDPI, Basel, Switzerland.
Note:
Related Files :
fluorescein isothiocyanate
Immunoaffinity
Levonorgestrel
Sol-gel
TMOS
Show More
Related Content
More details
DOI :
10.3390/ma4030469
Article number:
Affiliations:
Database:
Scopus
Publication Type:
article
;
.
Language:
English
Editors' remarks:
ID:
31292
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:01
Scientific Publication
Sol-gel entrapped levonorgestrel antibodies: Activity and structural changes as a function of different polymer formats
4
Shale, M., Department of Entomology, Institute of Plant Protection, The Volcani Center, Bet Dagan 50250, Israel
Miriam, A., Department of Entomology, Institute of Plant Protection, The Volcani Center, Bet Dagan 50250, Israel
Sol-gel entrapped levonorgestrel antibodies: Activity and structural changes as a function of different polymer formats
The paper describes development of a sol-gel based immunoaffinity method for the steroid hormone levonorgestrel (LNG) and the effects of changes in the sol-gel matrix format on the activity of the entrapped antibodies (Abs) and on matrix structure. The best sol-gel format for Ab entrapment was found to be a tetramethoxysilane (TMOS) based matrix at a TMOS:water ratio of 1:8, containing 10% polyethylene glycol (PEG) of MW 0.4 kDa. Addition of higher percentages of PEG or a higher MW PEG did not improve activity. No activity was obtained with a TMOS:water ratio of 1:12, most likely because of the very dense polymer that resulted from these polymerization conditions. Only minor differences in the non-specific binding were obtained with the various formats. TMOS was found to be more effective than tetrakis (2-hydroxyethyl)orthosilicate (THEOS) for entrapment of anti-levonorgestrel (LNG) Abs. However, aging the THEOS-based sol-gel for a few weeks at 4 °C stabilized the entrapped Abs and increased its binding capacity. Confocal fluorescent microscopy with fluorescein isothiocyanate (FITC) labeled immunoglobulines (IgGs) entrapped in the sol-gel matrix showed that the entrapped Abs were distributed homogenously within the gel. Scanning electron microscopy (SEM) images have shown the diverse structures of the various sol-gel formats and precursors. © 2011 by the authors; licensee MDPI, Basel, Switzerland.
Scientific Publication
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